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His Tag Anticorps

(His Tag)

His Tag Antibodies are commonly used in molecular biology and protein research. The His tag, which consists of a short sequence of histidine amino acids, is often added to recombinant proteins during their expression in host cells. This tag allows for easy purification and detection of the protein of interest using affinity chromatography and antibody-based assays. Here are some common applications of antibodies that detect His tags:

Protein Purification: His-tagged proteins can be purified using a technique called immobilized metal affinity chromatography (IMAC). In this method, a column containing metal ions (usually nickel or cobalt) is used to capture His-tagged proteins, which bind to the metal ions through the histidine residues. Antibodies that recognize the His tag can also be used in the purification process to ensure the specificity of the captured protein.
Protein Detection: Antibodies that specifically bind to His tags are used in techniques such as Western blotting and ELISA to detect the presence of His-tagged proteins in a sample. The antibody is typically conjugated with a detectable marker (such as an enzyme or a fluorescent molecule) that allows visualization and quantification of the target protein.

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His Tag anticorps

Reactivité: Humain WB Hôte: Souris Monoclonal 6AT18 unconjugated

Anti-HIS Tag at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 μg per lane.

Anti-HIS Tag at 1:8000 dilution + 12-tag lysate Lysates/proteins at 20 ng per lane.

Western blot analysis of lysates from 12tag protein , This demonstrates the His tagged antibody detected the His tagged protein (arrow).

3 images

38 références

N° du produit ABIN387750

His Tag anticorps

Rockland 600-401-382 WB, ELISA, IHC, DB Hôte: Lapin Polyclonal unconjugated

A knock-in mouse line with NALCN tagged with GFP, HA, and His tags (NALCN-GFP-HA-His mice).

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot.

Western blot of Rabbit Anti-6xHIS Epitope Tag antibody.

Crude extracts prepared from Salmonella strains with the phoR-8×Myc gene expressing PhoU-His, PhoUR184A-His, PhoUR184G-His, or the empty vector (pBAD33) were detected with anti-Myc (top) and anti-His (bottom) antibodies.

$Eluted fractions prepared from strains listed above were detected with anti-Myc (top) and anti-His (bottom) antibodies after immunoprecipitation with anti-Myc antibody-coated beads.$ WB

5 images

14 références

N° du produit ABIN100492

Rockland Original

His Tag anticorps

WB, ELISA Hôte: Lapin Polyclonal unconjugated

Western blot analysis of His-fusion protein (MW~33 kD) using 1 µg/mL Rabbit Anti-His-tag Polyclonal Antibody (ABIN398410) Lane 1-3: N-terminal His-fusion proteinLane 4: Negative E.

Western blot analysis of His-fusion protein (MW~91 kD) using 1 µg/mL Rabbit Anti-His-tag Polyclonal Antibody (ABIN398410) Lane 1: His-tag fusion protein expressed in E.

Western blot analysis of His-fusion protein (MW~91 kD) using 1 µg/mL Rabbit Anti-His-tag Polyclonal Antibody (ABIN398410) Lane 1-3: C-terminal His-fusion protein pfu-HisLane 4: Negative E.

3 images

16 références

N° du produit ABIN1573881

His Tag anticorps

WB, ELISA, IP, IF, FACS Hôte: Souris Monoclonal 6G2A9 unconjugated

Lane 1-3: Multiple Tag Cell Lysate (ABIN1536505) Lane 4: Negative controlPrimary Antibody: 1 µg/mL Anti-His Monoclonal Antibody (ABIN387699) Secondary Antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (ABIN398387, 1: 10,000)

Lane 1: 6x His Protein Ladder.

3 images

30 références

N° du produit ABIN387699

His Tag anticorps

Rockland 200-301-382 WB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2-G8 unconjugated

Characterization of purity and antigenicity of CKAP4 constructs.

Western Blot of Mouse anti-6xHIS Tag Antibody.

Western Blot using Immunochemicals' Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3).

ELISA of Mouse anti-6xHIS Tag Antibody.

4 images

4 références

N° du produit ABIN964552

Rockland Original

His Tag anticorps

Reactivité: Humain WB Hôte: Lapin Polyclonal RB19960 unconjugated

All lanes : Anti-His Tag Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: 293T/17 transfected with 12tag whole cell lysate Lane 3: 12tag recombinant protein Lysates/proteins at 1 μg per lane.

Anti-His Tag at 1:8000 dilution + 12tag recombination protein Lysates/proteins at 20 ng per lane.

2 images

1 reference

N° du produit ABIN652278

His Tag anticorps

WB, ELISA, IP, DB, ISt Hôte: Souris Monoclonal HIS-H8 unconjugated

Standard ladder containing five different His-tagged proteins, untransfected control (1), HEK293 cells transfected with His-tagged protein vector (2)

LEFT: untransfected control, RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells.

Comparison between anti-His tag (HIS.

3 images

5 références

N° du produit ABIN559683

His Tag anticorps (Cy3)

Rockland 200-304-382 WB, ELISA, FACS, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 Cy3

Western Blot of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

Western Blot using Immunochemicals' Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3).

3 images

3 références

N° du produit ABIN6720721

Rockland Original

His Tag anticorps (DyLight 800)

Rockland 200-345-382 WB, ELISA, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 DyLight 800

Western Blot of Mouse anti-6xHIS Tag Antibody.

Western Blot showing detection of 6x HIS Epitope Tag.

ELISA of Mouse anti-6xHIS Tag Antibody.

3 images

1 reference

N° du produit ABIN6720719

Rockland Original

His Tag anticorps (DyLight 649)

Rockland 200-343-382 WB, ELISA, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 DyLight 649

Western Blot of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

Western Blot of Mouse Anti-6X HIS EPITOPE TAG Antibody 649 Conjugated.

3 images

5 références

N° du produit ABIN6720637

Rockland Original

His Tag anticorps (HRP)

Rockland 200-303-382 WB, ELISA, IHC Hôte: Souris Monoclonal 33D10-D2-G8 HRP

Western Blot of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

3 images

1 reference

N° du produit ABIN964554

Rockland Original

His Tag anticorps

WB, ELISA Hôte: Souris Monoclonal 3G5H8 unconjugated

Western Blot Positive WB detected in: HIS fusion protein 1 at 20 ng, 10 ng, 5 ng, 2.

Western Blot Positive WB detected in: HIS fusion protein 2 at 20 ng, 10 ng, 5 ng, 2.

2 images

N° du produit ABIN7131650

His Tag anticorps

WB, IP, IF, IC Hôte: Souris Monoclonal unconjugated

Western blot analysis of over-expressed His-tagged protein in 293T cell lysate.

Immunoprecipitation of His-tagged protein from HEK293T cells transfected with vector overexpressing His tag, using Anti-His-tag Antibody.

Immunofluorescent analysis of His-tag staining in 293T cells transfected with a His-tag protein.

3 images

N° du produit ABIN7304940

His Tag anticorps (Cy5)

Rockland 200-310-382 FACS, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 Cy5

Western Blot of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

Western Blot using Immunochemicals' Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3).

3 images

N° du produit ABIN6720722

Rockland Original

His Tag anticorps (Biotin)

Rockland 200-306-382 WB, ELISA, IHC Hôte: Souris Monoclonal 33D10-D2-G8 Biotin

Western Blot of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

2 images

1 reference

N° du produit ABIN964555

Rockland Original

His Tag anticorps

Rockland 200-301-b90 WB, ELISA, IHC Hôte: Souris Monoclonal 33D10-D2-G8 unconjugated

Western Blot using Immunochemical's Mouse Anti-6x-His Epitope Tag Monoclonal Antibody showing detection of the 6xHis sequence on N-terminally-tagged (lane 2) and C-terminally-tagged recombinant proteins (lane 3).

Western Blot of Mouse anti-6xHIS Tag Antibody.

ELISA of Mouse anti-6xHIS Tag Antibody.

3 images

N° du produit ABIN1043732

Rockland Original

His Tag anticorps

Reactivité: Humain WB Hôte: Souris Monoclonal 6E6 unconjugated

Lane 1 and Lane 2: Recombinant human JSRV-ENV Protein Cell lysates; probed with His tag (6E6) Monoclonal Antibody, unconjugated (bsm-33004M) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

1 image

N° du produit ABIN5004651

His Tag anticorps

Reactivité: Tag, Souris, Rat, Bactéries WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

2 références

N° du produit ABIN727295

His Tag anticorps

Reactivité: Différentes espèces ELISA, IP, ICC, IHC (fro), CLIA Hôte: Souris Monoclonal D10 unconjugated

Detection of Recombinant Protein with His-tag using Monoclonal Antibody to Hexa Histidine (H6)

1 image

N° du produit ABIN7426805

His Tag anticorps

WB, ELISA, IP, IHC, IF Hôte: Souris Monoclonal HIS-H8 unconjugated

1 reference

N° du produit ABIN2443717

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