BMP7 Kit ELISA

N° du produit ABIN1112568

Détail du produit BMP7 Kit ELISA

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: For quantitative detection of BMP-7 in human serum, plasma, urine, bone tissue or cell culture supernatants.

Type d'échantillon: Serum, Plasma, Urine, Tissue Samples, Cell Culture Supernatant

Analytical Method: Quantitative

Sensibilité: < 10 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Human BMP-7 antibody 2. Lyophilized Human BMP-7 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Human BMP-7 antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BMP-7 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BMP-7 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the BMP-7 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of BMP-7 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Bone Tissue: Extract demineralized bone samples in 4 M Guanidine-HCl and protease inhibitors. Dissolve the final sample in 2 M Guanidine-HCl.
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 1000 × g for 15 min. Analyze the serum immediately or aliquot and store at -20 °C .
Plasma: Collect plasma with heparin or EDTA as the anticoagulant. Centrifuge for 15min at 2-8°C at 1500 x g within 30 min of collection. Analyze immediately or aliquot and store frozen at -20 °C. Citrate can not be used as anticoagulant here.
Urine: Aseptically collect the first urine of the day, micturate directly into a sterile container. Remove particular impurities by centrifugation, assay immediately or aliquot and store samples at -20 °C. Note: 1. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 2. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP-7 (BMP7 Produits)

Synonymes: bmp-7 Kit ELISA, bmp7 Kit ELISA, op-1 Kit ELISA, BMP7 Kit ELISA, zgc:153025 Kit ELISA, OP-1 Kit ELISA, OP1 Kit ELISA, BMP-7 Kit ELISA, fb83f09 Kit ELISA, wu:fb83f09 Kit ELISA, bone morphogenetic protein 7 Kit ELISA, bone morphogenetic protein 7, gene 1 L homeolog Kit ELISA, bone morphogenetic protein 7b Kit ELISA, bone morphogenetic protein 7, gene 2 L homeolog Kit ELISA, bone morphogenetic protein 7a Kit ELISA, BMP7 Kit ELISA, bmp7.1.L Kit ELISA, LOC100136282 Kit ELISA, bmp7b Kit ELISA, Bmp7 Kit ELISA, LOC100008826 Kit ELISA, bmp7.2.L Kit ELISA, bmp7a Kit ELISA

Sujet: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a member of the TGF-beta superfamily. It is expressed in the brain, kidneys and bladder. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 induces the phosphorylation of SMAD1 and SMAD5, which in turn induce transcription of numerous osteogenic genes. It has been demonstrated that BMP7 treatment is sufficient to induce all of the genetic markers of osteoblast differentiation in many cell types.

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance