BMP7 Kit ELISA

N° du produit ABIN1672823

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain BMP7. Il a été cité dans 5 publications.

Aperçu rapide pour BMP7 Kit ELISA (ABIN1672823)

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Épitope: AA 293-431

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Osseous Tissue, Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine

Détail du produit BMP7 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Human BMP-7 ELISA Kit to quantitate Human BMP7 in bone tissue, cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: S293-H431

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.3%, 4.7%, 5.5%
Inter-Assay Precision (CV%): 8.8%, 6.5%, 6.1%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour BMP7 Kit ELISA (ABIN1672823)

Wang, Xue, Zhao, Liu, Ma, Ma: "Form-deprivation myopia induces decreased expression of bone morphogenetic protein-2, 5 in guinea pig sclera." dans: International journal of ophthalmology, Vol. 8, Issue 1, pp. 39-45, (2015) (PubMed).

Li, Yi, Jin, Duan, Min: "Effects of sequentially released BMP-2 and BMP-7 from PELA microcapsule-based scaffolds on the bone regeneration." dans: American journal of translational research, Vol. 7, Issue 8, pp. 1417-28, (2015) (PubMed).

Qu, Du, Zhang, Li, Han, Qiu: "Changes in the expression of bone morphogenetic protein 7 and tamm- horsfall protein in the early stages of diabetic nephropathy." dans: Nephro-urology monthly, Vol. 4, Issue 2, pp. 466-9, (2013) (PubMed).

Tu, Dong, Lu: "Effects of Panax notoginoside on the nephropathy in rats with type 1 diabetes mellitus." dans: Chinese journal of integrative medicine, Vol. 17, Issue 8, pp. 612-5, (2011) (PubMed).

Che, Zhang, Li, Tan, Bai, Qu: "Application of tissue-engineered cartilage with BMP-7 gene to repair knee joint cartilage injury in rabbits." dans: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA, Vol. 18, Issue 4, pp. 496-503, (2010) (PubMed).

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP7

Sujet:

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Gene Full Name: bone morphogenetic protein 7

ID gène: 655

UniProt: P18075

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance