PPT1 Kit ELISA

N° du produit ABIN1146897

Le kit ELISA PPT1 (ABIN1146897) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain PPT1.

Aperçu rapide pour PPT1 Kit ELISA (ABIN1146897)

Antigène: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Gamme de detection: 0.62-40.0 ng/mL

Application: ELISA

Détail du produit PPT1 Kit ELISA

Seuil minimal de détection: 0.62 ng/mL

Attributs du produit: The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PPT-1. During the reaction, PPT-1 in the sample or standard competes with a fixed amount of biotin-labeled PPT-1 for sites on a pre-coated Monoclonal antibody specific to PPT-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PPT-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Ingrédients: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A (1x120µl), Detection Reagent B (1x120µl), Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Information d'application

Commentaires:

Gene Name: PPT1

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: PPT1 (Palmitoyl-Protein Thioesterase 1 (PPT1))

Autre désignation: Palmitoyl-protein thioesterase 1

Sujet: Synonyms: Homo sapiens,Human,Palmitoyl-protein hydrolase 1,Palmitoyl-protein thioesterase 1,PPT,PPT1,PPT-1

Pathways: SARS-CoV-2 Protein Interactome