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DDO Kit ELISA

DDO Reactivité: Humain Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN1569370
  • Antigène Voir toutes DDO Kits ELISA
    DDO (D-Aspartate Oxidase (DDO))
    Reactivité
    • 3
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DDO in human tissue homogenates and other biological fluids.
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of this index.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
    Sensibilité
    0.059 ng/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Standard (freeze dried)
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • TMB
    • Stop Solution
    • Wash Buffer (30X)
    • Plate sealer for 96 wells
    • Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Volume d'échantillon
    100 μL
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Procédure de l'essai

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Précision du teste
    • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
    • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
    • CV(%) = SD/meanX100
    • Intra-assay: CV<10%
    • Inter-assay: CV<12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
    Date de péremption
    12 months
  • Antigène Voir toutes DDO Kits ELISA
    DDO (D-Aspartate Oxidase (DDO))
    Autre désignation
    DDO (DDO Produits)
    Synonymes
    DDO Kit ELISA, DDBDRAFT_0203041 Kit ELISA, DDBDRAFT_0238431 Kit ELISA, DDB_0203041 Kit ELISA, DDB_0238431 Kit ELISA, DDBDRAFT_0217305 Kit ELISA, DDBDRAFT_0238432 Kit ELISA, DDB_0217305 Kit ELISA, DDB_0238432 Kit ELISA, 5330420D20Rik Kit ELISA, 5730402C02Rik Kit ELISA, AI467244 Kit ELISA, DASOX Kit ELISA, DDO-1 Kit ELISA, DDO-2 Kit ELISA, D-aspartate oxidase Kit ELISA, D-amino-acid:oxygen oxidoreductase Kit ELISA, DDO Kit ELISA, IFG3 Kit ELISA, LACBIDRAFT_308911 Kit ELISA, PAS_chr3_0406 Kit ELISA, Amir_4180 Kit ELISA, Gbro_4657 Kit ELISA, ddo-2 Kit ELISA, ddo-1 Kit ELISA, Sros_8818 Kit ELISA, Kfla_2239 Kit ELISA, Gobs_3505 Kit ELISA, Snas_1168 Kit ELISA, ddo Kit ELISA, Ddo Kit ELISA
    Sujet
    Alternative name: DASOX, DDO-1, DDO-2
    ID gène
    8528
    UniProt
    Q99489
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