DDO Kit ELISA

N° du produit ABIN5653541

Détail du produit DDO Kit ELISA

Antigène: DDO (D-Aspartate Oxidase (DDO))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of D-Aspartate Oxidase (DDO). No significant cross-reactivity or interference between D-Aspartate Oxidase (DDO) and analogues was observed.

Sensibilité: 0.059 ng/mL

Information d'application

Commentaires:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Durée du test: 3 h

Plaque: Pre-coated

Protocole: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-Aspartate Oxidase (DDO). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to D-Aspartate Oxidase (DDO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain D-Aspartate Oxidase (DDO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Aspartate Oxidase (DDO) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level D-Aspartate Oxidase (DDO) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level D-Aspartate Oxidase (DDO) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Stock: 4 °C,-20 °C

Stockage commentaire: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Date de péremption: 4-8 months

Détail du antigène

Antigène: DDO (D-Aspartate Oxidase (DDO))

Autre désignation: D-Aspartate Oxidase (DDO Produits)

Synonymes: DDO Kit ELISA, DDBDRAFT_0203041 Kit ELISA, DDBDRAFT_0238431 Kit ELISA, DDB_0203041 Kit ELISA, DDB_0238431 Kit ELISA, DDBDRAFT_0217305 Kit ELISA, DDBDRAFT_0238432 Kit ELISA, DDB_0217305 Kit ELISA, DDB_0238432 Kit ELISA, 5330420D20Rik Kit ELISA, 5730402C02Rik Kit ELISA, AI467244 Kit ELISA, DASOX Kit ELISA, DDO-1 Kit ELISA, DDO-2 Kit ELISA, D-aspartate oxidase Kit ELISA, D-amino-acid:oxygen oxidoreductase Kit ELISA, DDO Kit ELISA, IFG3 Kit ELISA, LACBIDRAFT_308911 Kit ELISA, PAS_chr3_0406 Kit ELISA, Amir_4180 Kit ELISA, Gbro_4657 Kit ELISA, ddo-2 Kit ELISA, ddo-1 Kit ELISA, Sros_8818 Kit ELISA, Kfla_2239 Kit ELISA, Gobs_3505 Kit ELISA, Snas_1168 Kit ELISA, ddo Kit ELISA, Ddo Kit ELISA

Sujet:

Gene Name: D-Aspartate Oxidase

Gene Aliases: DASOX, DDO-1, DDO-2

UniProt: Q99489