HMOX1 Kit ELISA

N° du produit ABIN1570329

Détail du produit HMOX1 Kit ELISA

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.625 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.625 pg/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HO1 in mouse serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 6.3 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: HMOX1 (Heme Oxygenase (Decycling) 1 (HMOX1))

Autre désignation: HO1 (HMOX1 Produits)

Synonymes: HMOX1 Kit ELISA, ARABIDOPSIS THALIANA HEME OXYGENASE 1 Kit ELISA, ATHO1 Kit ELISA, F18A8.4 Kit ELISA, F18A8_4 Kit ELISA, GENOMES UNCOUPLED 2 Kit ELISA, GUN2 Kit ELISA, HEME OXYGENASE Kit ELISA, HEME OXYGENASE 1 Kit ELISA, HEME OXYGENASE 6 Kit ELISA, HO1 Kit ELISA, HY1 Kit ELISA, HY6 Kit ELISA, PLASTID HEME OXYGENASE Kit ELISA, REVERSAL OF THE DET PHENOTYPE 4 Kit ELISA, HO-1 Kit ELISA, wu:fc27c04 Kit ELISA, zgc:65984 Kit ELISA, D8Wsu38e Kit ELISA, Hemox Kit ELISA, Hmox Kit ELISA, Hsp32 Kit ELISA, HEOXG Kit ELISA, Heox Kit ELISA, Ho-1 Kit ELISA, Ho1 Kit ELISA, hsp32 Kit ELISA, MGC132176 Kit ELISA, Hmox1 Kit ELISA, HMOX1D Kit ELISA, HSP32 Kit ELISA, bK286B10 Kit ELISA, heme oxygenase 1 Kit ELISA, Plant heme oxygenase (decyclizing) family protein Kit ELISA, Heme oxygenase Kit ELISA, heme oxygenase Kit ELISA, heme oxygenase 1, chloroplastic Kit ELISA, heme oxygenase 1a Kit ELISA, heme oxygenase 1 L homeolog Kit ELISA, HMOX1 Kit ELISA, TED4 Kit ELISA, ho1 Kit ELISA, P9301_RS17555 Kit ELISA, A9601_RS17590 Kit ELISA, MAE_RS06565 Kit ELISA, HO1 Kit ELISA, hmox1a Kit ELISA, CPE0214 Kit ELISA, Cyan7425_2962 Kit ELISA, Hmox1 Kit ELISA, hmox1.L Kit ELISA, AM1_C0205 Kit ELISA, CC1G_11686 Kit ELISA, CpipJ_CPIJ007246 Kit ELISA

Sujet: Alternative name: HMOX1, Hsp32, HMOX1D

ID gène: 15368

UniProt: P14901

Pathways: Transition Metal Ion Homeostasis, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome