14-3-3 zeta Kit ELISA

N° du produit ABIN1573149

Détail du produit 14-3-3 zeta Kit ELISA

Antigène: 14-3-3 zeta (YWHAZ)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of YWHAz in human serum, plasma, tissue homogenates, cell lysates and other biological fluids.

Type d'échantillon: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of this index.

Réactivité croisée (Details): No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.

Sensibilité: 0.053 ng/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate
• Standard (freeze dried)
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• TMB
• Stop Solution
• Wash Buffer (30X)
• Plate sealer for 96 wells
• Instruction manual

Matériel non inclus:

1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Procédure de l'essai:

The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Précision du teste:

• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
• CV(%) = SD/meanX100
• Intra-assay: CV<10%
• Inter-assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.

Stock: 4 °C,-20 °C

Stockage commentaire: The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.

Date de péremption: 12 months

Détail du antigène

Antigène: 14-3-3 zeta (YWHAZ)

Autre désignation: YWHAz (YWHAZ Produits)

Synonymes: 14-3-3-zeta Kit ELISA, KCIP-1 Kit ELISA, YWHAD Kit ELISA, 14-3-3zeta Kit ELISA, Ywhaz Kit ELISA, ACYPI003154 Kit ELISA, 14-3-3z Kit ELISA, kcip-1 Kit ELISA, ywhaq Kit ELISA, 1433z Kit ELISA, ywhaz Kit ELISA, ywhazb Kit ELISA, 1110013I11Rik Kit ELISA, AI596267 Kit ELISA, AL022924 Kit ELISA, AU020854 Kit ELISA, ywhaza Kit ELISA, fb14h09 Kit ELISA, wu:fb05g08 Kit ELISA, wu:fb14h09 Kit ELISA, ywhai Kit ELISA, zgc:55807 Kit ELISA, 14-3-3 Kit ELISA, 14-3-3 zeta Kit ELISA, 14-3-3ZETA Kit ELISA, 14-3-3leo Kit ELISA, 2G1 Kit ELISA, 4-3-3 zeta Kit ELISA, 5.11 Kit ELISA, 549 Kit ELISA, BEST:GH05075 Kit ELISA, CG17870 Kit ELISA, D14-3-3 Kit ELISA, D14-3-3zeta Kit ELISA, Dmel\\CG17870 Kit ELISA, K Kit ELISA, LEO Kit ELISA, Leo Kit ELISA, PAR-5 Kit ELISA, PAR5 Kit ELISA, Par-5 Kit ELISA, d14-3-3zeta Kit ELISA, l(2)07103 Kit ELISA, l(2)46CFe Kit ELISA, l(2)46Ee Kit ELISA, leo Kit ELISA, par-5 Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta Kit ELISA, 14-3-3 protein zeta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta Kit ELISA, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog Kit ELISA, 14-3-3 protein zeta/delta pseudogene Kit ELISA, CG17870 gene product from transcript CG17870-RE Kit ELISA, YWHAZ Kit ELISA, 14-3-3zeta Kit ELISA, ywhaz Kit ELISA, 1433z Kit ELISA, ywhaz.L Kit ELISA, Ywhaz Kit ELISA, ywhaz.S Kit ELISA, LOC100855903 Kit ELISA

Sujet: Alternative name: KCIP-1, 14-3-3-zeta, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1

ID gène: 7534

UniProt: P63104

Pathways: Apoptose, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location