Angiopoietin 2 Kit ELISA

N° du produit ABIN1672763

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain Angiopoietin 2. Il y a 1 publication disponible.

Aperçu rapide pour Angiopoietin 2 Kit ELISA (ABIN1672763)

Antigène: Angiopoietin 2 (ANGPT2)

Épitope: AA 19-496

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Milk

Détail du produit Angiopoietin 2 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Angiopoietin-2

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Y19-F496

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Y19-F496

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 fibrinogen C-terminal domain.

Plaque: Pre-coated

Protocole: human Angiopoietin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Angiopoietin-2 has been precoated onto 96-well plates. Standards(NSO, Y19-F496) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Angiopoietin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Angiopoietin-2 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Angiopoietin-2 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 1.08, Standard deviation: 0.049, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 3.49, Standard deviation: 0.181, CV(%): 5.2
• Sample 3: n=16, Mean(ng/ml): 7.01, Standard deviation: 0.407, CV(%): 5.8,
• Sample 1: n=24, Mean(ng/ml): 1.05, Standard deviation: 0.071, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 3.32, Standard deviation: 0.236, CV(%): 7.1
• Sample 3: n=24, Mean(ng/ml): 6.67, Standard deviation: 0.5, CV(%): 7.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour Angiopoietin 2 Kit ELISA (ABIN1672763)

You, Zhuge, Zhu, Si et al.: "Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with ..." dans: International journal of ophthalmology, Vol. 7, Issue 4, pp. 648-53, (2014) (PubMed).

Détail du antigène

Antigène: Angiopoietin 2 (ANGPT2)

Autre désignation: ANGPT2

Sujet:

Protein Function: Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. .

Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Synonyms: Angiopoietin-2,ANG-2,ANGPT2,

Full Gene Name: Angiopoietin-2

Cellular Localisation: Secreted.

ID gène: 285

UniProt: O15123

Pathways: Signalisation RTK