CCL17 Kit ELISA

N° du produit ABIN1672770

Ce kit ELISA Souris CCL17 est un kit ELISA Colorimetric conçu pour quantifier Souris CCL17.

Aperçu rapide pour CCL17 Kit ELISA (ABIN1672770)

Antigène: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Épitope: AA 24-93

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit CCL17 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CCL17/TARC

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A24-P93

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A24-P93

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the intercrine beta (chemokine CC) family.

Plaque: Pre-coated

Protocole: mouse CCL17 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CCL17 has been precoated onto 96-well plates. Standards(E.coli, A24-P93) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL17 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CCL17 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CCL17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CCL17 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.29, CV(%): 4.9
• Sample 2: n=16, Mean(pg/ml): 437, Standard deviation: 23.16, CV(%): 5.3
• Sample 3: n=16, Mean(pg/ml): 1294, Standard deviation: 82.82, CV(%): 6.4,
• Sample 1: n=24, Mean(pg/ml): 113, Standard deviation: 9.27, CV(%): 8.2
• Sample 2: n=24, Mean(pg/ml): 472, Standard deviation: 40.59, CV(%): 8.6
• Sample 3: n=24, Mean(pg/ml): 1302, Standard deviation: 118.48, CV(%): 9.1

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Autre désignation: CCL17

Sujet:

Background: Chemokine(C-C motif) ligand 17(CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine(TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells. This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.

Synonyms: C-C motif chemokine ,Ccl17 ,

Full Gene Name: C-C motif chemokine

Cellular Localisation: Secreted.

ID gène: 20295

UniProt: F6R5P4