c-MET Kit ELISA

N° du produit ABIN1672789

Kit ELISA Souris c-MET, test Colorimetric pour la quantification de Souris c-MET.

Aperçu rapide pour c-MET Kit ELISA (ABIN1672789)

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Épitope: AA 25-306, AA 307-929

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit c-MET Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: For quantitative detection of mouse C-MET in cell culture supernates, serum and plasma(heparin, EDTA).

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Natural and recombinant mouse C-MET

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <5pg/mL

Ingrédients:

• 96-well plate precoated with anti- mouse C-MET antibod - 1
• Lyophilized recombinant mouse C-MET standar - 10ng/tubex2
• Biotinylated anti- mouse C-MET antibod - 130μl(dilution 1:100)
• Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
• Sample diluent buffe - 30 ml
• Antibody diluent buffe - 12ml
• ABC diluent buffe - 12ml
• TMB color developing agen - 10ml
• TMB stop solutio - 10ml

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: E25-R306(alpha)&S307-N929(beta)

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse C-MET ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for C-MET has been precoated onto 96-well plates. Standards(sf21, E25-R306(α) &S307-N929(β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for C-MET is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse C-MET amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse C-MET standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse C-MET standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.51, Standard deviation: 0.022, CV(%): 4.3
• Sample 2: n=16, Mean(ng/ml): 1.7, Standard deviation: 0.092, CV(%): 5.4
• Sample 3: n=16, Mean(ng/ml): 2.8, Standard deviation: 0.146, CV(%): 5.2,
• Sample 1: n=24, Mean(ng/ml): 0.57, Standard deviation: 0.029, CV(%): 5.1
• Sample 2: n=24, Mean(ng/ml): 1.8, Standard deviation: 0.137, CV(%): 7.6
• Sample 3: n=24, Mean(ng/ml): 3.2, Standard deviation: 0.221, CV(%): 6.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Autre désignation: MET

Sujet: C-Met(MET or MNNG HOS Transforming gene) is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor(HGFR). MET proto-oncogene has a total length of 125,982 bp, and it is located in the 7q31 locus of chromosome 7. MET is a membrane receptor that is essential for embryonic development and wound healing. Activation of MET triggers mitogenesis, and morphogenesis.

ID gène: 17295

UniProt: A4FUV6

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor