c-MET Kit ELISA

N° du produit ABIN1672789

Kit ELISA c-MET Souris, Colorimetric test pour la quantification de Souris c-MET.

Aperçu rapide pour c-MET Kit ELISA (ABIN1672789)

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit c-MET Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Sandwich Mouse HGFR/c-MET ELISA Kit to quantitate Mouse MET in cell culture supernatants,serum,plasma(heparin, EDTA).

Analytical Method: Quantitative

Specificité:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.0%, 3.1%, 2.7%
Inter-Assay Precision (CV%): 3.2%, 5.7%, 3.1%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Détail du antigène

Antigène: c-MET (MET) (Met Proto-Oncogene (MET))

Autre désignation: MET

Sujet:

Background: Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. Enables hepatocyte growth factor receptor activity. Involved in cell surface receptor signaling pathway, modulation of chemical synaptic transmission, and positive chemotaxis. Acts upstream of or within several processes, including hepatocyte growth factor receptor signaling pathway, regulation of cytokine production, and striated muscle cell differentiation. Located in membrane. Is expressed in several structures, including alimentary system, branchial arch, central nervous system, genitourinary system, and limb.

Gene Full Name: Hepatocyte growth factor receptor

ID gène: 17295

UniProt: P16056

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor