Galectin 3 Kit ELISA

N° du produit ABIN1672793

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris Galectin 3.

Aperçu rapide pour Galectin 3 Kit ELISA (ABIN1672793)

Antigène: Galectin 3 (LGALS3)

Épitope: AA 2-264

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit Galectin 3 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Galectin-3/LGALS3

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A2-I264

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: A2-I264

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse Galectin-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Galectin-3 has been precoated onto 96-well plates. Standards(E.coli, A2-I264) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Galectin-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Galectin-3 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Galectin-3 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 850, Standard deviation: 37.4, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 1347, Standard deviation: 66, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 2932, Standard deviation: 161.3, CV(%): 5.5,
• Sample 1: n=24, Mean(pg/ml): 795, Standard deviation: 54.06, CV(%): 6.8
• Sample 2: n=24, Mean(pg/ml): 1046, Standard deviation: 99.83, CV(%): 7.1
• Sample 3: n=24, Mean(pg/ml): 2758, Standard deviation: 201.3, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Galectin 3 (LGALS3)

Autre désignation: LGALS3

Classe de substances: Chemical

Sujet:

Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21â€"q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

Synonyms: Galectin ,Lgals3 ,mCG_6908 ,

Full Gene Name: Galectin

ID gène: 16854

UniProt: Q8C253

Pathways: Signalisation RTK