IL-21 Kit ELISA

N° du produit ABIN1672800

Détail du produit IL-21 Kit ELISA

Antigène: IL-21 (IL21) (Interleukin 21 (IL21))

Épitope: AA 25-146

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IL-21

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: P25-S146

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: P25-S146

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: mouse IL-21 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IL-21 has been precoated onto 96-well plates. Standards(E.coli, P25-S146) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-21 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IL-21 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IL-21 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 316, Standard deviation: 15.8, CV(%): 5
• Sample 2: n=16, Mean(pg/ml): 2196, Standard deviation: 80.4, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 2113, Standard deviation: 99.31, CV(%): 4.7,
• Sample 1: n=24, Mean(pg/ml): 427, Standard deviation: 25.2, CV(%): 5.9
• Sample 2: n=24, Mean(pg/ml): 1450, Standard deviation: 97.15, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 2485, Standard deviation: 159, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IL-21 Kit ELISA (ABIN1672800)

Li, Jin, Wang, Li, Liu, Chang, Wang, Liu: "Live attenuated Salmonella displaying HIV-1 10E8 epitope on fimbriae: systemic and mucosal immune responses in BALB/c mice by mucosal administration." dans: Scientific reports, Vol. 6, pp. 29556, (2018) (PubMed).

Shen, Yang, Yang, Wang, Cui, Zhou, Liu, Pan, Liu, Zhang, Zhang, Xie, Liu: "Hepatitis B virus persistence in mice reveals IL-21 and IL-33 as regulators of viral clearance." dans: Nature communications, Vol. 8, Issue 1, pp. 2119, (2017) (PubMed).

Yu, Tian, Wang, Guo, Li, Wang, Li, Zhang, Zhuang, Ling: "An HIV-1 envelope immunogen with W427S mutation in CD4 binding site induced more T follicular helper memory cells and reduced non-specific antibody responses." dans: PLoS ONE, Vol. 9, Issue 12, pp. e115047, (2014) (PubMed).

Détail du antigène

Antigène: IL-21 (IL21) (Interleukin 21 (IL21))

Autre désignation: IL21 (IL21 Produits)

Synonymes: IL-21 Kit ELISA, Za11 Kit ELISA, interleukin 21 Kit ELISA, IL21 Kit ELISA, Il21 Kit ELISA

Sujet:

Background: Interleukin-21 is a protein that in humans is encoded by the IL21 gene. Interleukin 21 is a cytokine that has potent regulatory effects on cells of the immune system, including natural killer(NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells. By radiation hybrid analysis, the IL21 gene is mapped to chromosome 4q26-q27, near the IL2 gene. IL21 stimulates B-cell proliferation in an anti-CD40-dependent manner but inhibits proliferation stimulated by IL4 plus anti-IgM. The biologic effects of IL21 on NK cells differ between species, with human IL21 enhancing NK cell proliferation and murine IL21 inhibiting NK cell proliferation. IL21 is a cytokine highly expressed by mouse T(H)17 cells.

Synonyms: Interleukin ,Il21 ,mCG_12674 ,

Full Gene Name: Interleukin

ID gène: 60505

UniProt: Q5SUE2

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response