BMP7 Kit ELISA

N° du produit ABIN6719712

N° du produit (Fournisseur): koa0871

Le kit ELISA BMP7 (ABIN6719712) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat BMP7.

Aperçu rapide pour BMP7 Kit ELISA (ABIN6719712)

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Osseous Tissue, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit BMP7 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0871

Fournisseur: Rockland

Fonction: Rat BMP-7 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: S292-H430

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each rat BMP-7 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 6.5%, 7.6%, 4.8%
Inter-Assay Precision (CV%): 6.2%, 8.9%, 6.5%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: BMP7 (Bone Morphogenetic Protein 7 (BMP7))

Autre désignation: BMP-7

Sujet:

Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Gene Full Name: bone morphogenetic protein 7

ID gène: 85272

UniProt: Q9R228

Pathways: Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance