COL18A1 Kit ELISA

N° du produit ABIN1672824

Ce kit ELISA Humain COL18A1 est un kit ELISA Colorimetric conçu pour quantifier Humain COL18A1. Ce Kit ELISA a été cité dans 2+ publications.

Aperçu rapide pour COL18A1 Kit ELISA (ABIN1672824)

Antigène: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Épitope: AA 1154-1335

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Milk

Détail du produit COL18A1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Endostatin

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: H1154-S1335

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: E.coli
Immunogen sequence: H1154-S1335

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the multiplexin collagen family.

Tissue Specificity: Present in multiple organs with highest levels in liver, lung and kidney.

Plaque: Pre-coated

Protocole: human Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Endostatin has been precoated onto 96-well plates. Standards(E.coli, H1154-S1335) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Endostatin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or human milk to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Endostatin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.75, Standard deviation: 0.054, CV(%): 7.2
• Sample 2: n=16, Mean(ng/ml): 2.59, Standard deviation: 0.168, CV(%): 6.5
• Sample 3: n=16, Mean(ng/ml): 5.21, Standard deviation: 0.224, CV(%): 4.3,
• Sample 1: n=24, Mean(ng/ml): 0.89, Standard deviation: 0.072, CV(%): 8.1
• Sample 2: n=24, Mean(ng/ml): 2.46, Standard deviation: 0.177, CV(%): 7.2
• Sample 3: n=24, Mean(ng/ml): 5.16, Standard deviation: 0.346, CV(%): 6.7

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour COL18A1 Kit ELISA (ABIN1672824)

Fu, Zhao, Wu, Wu, Xu, Liu, Tong: "Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure." dans: Journal of ethnopharmacology, Vol. 139, Issue 1, pp. 26-33, (2011) (PubMed).

Zhang, Qu, Cui, Guo, Zhang, Ma, Sun: "Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice." dans: Cancer biology & therapy, Vol. 8, Issue 5, pp. 466-73, (2009) (PubMed).

Détail du antigène

Antigène: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Autre désignation: Endostatin

Classe de substances: Chemical

Sujet:

Protein Function: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Synonyms: Collagen alpha-1(XVIII) chain,Endostatin,COL18A1,

Full Gene Name: Collagen alpha-1(XVIII) chain

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 80781

UniProt: P39060