GDNF Kit ELISA

N° du produit ABIN1672848

Le kit ELISA GDNF (ABIN1672848) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris GDNF. Ce kit ELISA a été cité dans 5 publications.

Aperçu rapide pour GDNF Kit ELISA (ABIN1672848)

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Épitope: AA 78-211

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit GDNF Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Mouse GDNF ELISA Kit to quantitate Mouse Gdnf in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard:NS0
Immunogen sequence: S78-I211

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.5%, 4.1%, 7%
Inter-Assay Precision (CV%): 7.6%, 4.2%, 7.8%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour GDNF Kit ELISA (ABIN1672848)

Song, Kong, Acosta, Sava, Borlongan, Sanchez-Ramos: "Granulocyte colony-stimulating factor promotes behavioral recovery in a mouse model of traumatic brain injury." dans: Journal of neuroscience research, Vol. 94, Issue 5, pp. 409-23, (2016) (PubMed).

Banerjee, Nürnberger, Hennerbichler, Riedl, Schuh, Hacobian, Teuschl, Eibl, Redl, Wolbank: "In toto differentiation of human amniotic membrane towards the Schwann cell lineage." dans: Cell and tissue banking, Vol. 15, Issue 2, pp. 227-39, (2014) (PubMed).

Liu, Wang, Shao, Liu: "Genetically modified Schwann cells producing glial cell line-derived neurotrophic factor inhibit neuronal apoptosis in rat spinal cord injury." dans: Molecular medicine reports, Vol. 9, Issue 4, pp. 1305-12, (2014) (PubMed).

Chai, Guo, Li, Wang, Wang, Shi, Hu, Liu, Adah: "Scutellarin and caffeic acid ester fraction, active components of Dengzhanxixin injection, upregulate neurotrophins synthesis and release in hypoxia/reoxygenation rat astrocytes." dans: Journal of ethnopharmacology, Vol. 150, Issue 1, pp. 100-7, (2013) (PubMed).

Yang, Zhou, Gao, Chen, Tu, Sun, Liu, He, Liu, Yuan: "Neuroprotective effects of bone marrow stem cells overexpressing glial cell line-derived neurotrophic factor on rats with intracerebral hemorrhage and neurons exposed to hypoxia/reoxygenation." dans: Neurosurgery, Vol. 68, Issue 3, pp. 691-704, (2011) (PubMed).

Détail du antigène

Antigène: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Autre désignation: GDNF

Sujet:

Background: Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily.1 GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures.2 GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.

Gene Full Name: glial cell line derived neurotrophic factor

ID gène: 14573

UniProt: P48540

Pathways: Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration