alpha 2 Macroglobulin Kit ELISA

N° du produit ABIN1672904

Détail du produit alpha 2 Macroglobulin Kit ELISA

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625-40.000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human A2M/alpha2-Macroglobulin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: from human plasma

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: from human plasma

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Tissue Specificity: Secreted in plasma. .

Plaque: Pre-coated

Protocole: human A2M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for A2M has been precoated onto 96-well plates. Standards(from human plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for A2M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human A2M amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human A2M standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human A2M standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 6.18, Standard deviation: 0.377, CV(%): 6.1
• Sample 2: n=16, Mean(ng/ml): 12.04, Standard deviation: 0.771, CV(%): 6.4
• Sample 3: n=16, Mean(ng/ml): 25.21, Standard deviation: 0.174, CV(%): 6.9,
• Sample 1: n=24, Mean(ng/ml): 6.72, Standard deviation: 0.457, CV(%): 6.8
• Sample 2: n=24, Mean(ng/ml): 13.68, Standard deviation: 0.985, CV(%): 7.2
• Sample 3: n=24, Mean(ng/ml): 26.81, Standard deviation: 2.12, CV(%): 7.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour alpha 2 Macroglobulin Kit ELISA (ABIN1672904)

Liu, Chen, Wu, Chen, Zhang: "Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients." dans: Anatolian journal of cardiology, Vol. 16, Issue 1, pp. 23-8, (2016) (PubMed).

Wu, Liu, Chen, Chen, Zhang: "Disequilibrium of Blood Coagulation and Fibrinolytic System in Patients With Coronary Artery Ectasia." dans: Medicine, Vol. 95, Issue 8, pp. e2779, (2016) (PubMed).

Détail du antigène

Antigène: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Autre désignation: A2M (A2M Produits)

Synonymes: A2MD Kit ELISA, CPAMD5 Kit ELISA, FWP007 Kit ELISA, S863-7 Kit ELISA, A2MAC1 Kit ELISA, A2m1 Kit ELISA, A2maa Kit ELISA, Mam Kit ELISA, A2mp Kit ELISA, A2M Kit ELISA, LOC733429 Kit ELISA, endod Kit ELISA, cpamd5 Kit ELISA, fwp007 Kit ELISA, s863-7 Kit ELISA, a2mb Kit ELISA, endodermin Kit ELISA, Alpha-2-M Kit ELISA, alpha-2-macroglobulin Kit ELISA, pregnancy-zone protein L homeolog Kit ELISA, alpha-2-macroglobulin S homeolog Kit ELISA, pregnancy zone protein Kit ELISA, alpha-2-macroglobulin-like Kit ELISA, A2M Kit ELISA, A2m Kit ELISA, LOC477699 Kit ELISA, pzp.L Kit ELISA, AZL_c00450 Kit ELISA, LOC100349077 Kit ELISA, a2m Kit ELISA, LOC100061656 Kit ELISA, LOC100090399 Kit ELISA, a2m.S Kit ELISA, LOC100469973 Kit ELISA, LOC100595735 Kit ELISA, PZP Kit ELISA, LOC100353095 Kit ELISA, LOC101122940 Kit ELISA, LOC101801552 Kit ELISA, LOC100911545 Kit ELISA

Sujet:

Protein Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Background: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease(AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates(activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

Synonyms: Alpha-2-macroglobulin,Alpha-2-M,C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5,A2M,CPAMD5,FWP007,

Full Gene Name: Alpha-2-macroglobulin

Cellular Localisation: Secreted.

ID gène: 2

UniProt: P01023

Pathways: Lipid Metabolism