SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity

N° du produit ABIN1882520

Aperçu rapide pour SensoLyte® Plus 520 MMP-2 Assay Kit Fluorimetric and Enhanced Selectivity (ABIN1882520)

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivité: Humain

Méthode de détection: Fluorometric

Application: Fluorescence Resonance Energy Transfer Microscopy (FRET)

Détail du produit

Attributs du produit: The SensoLyte® Plus 520 MMP-2 Assay Kit is designed specifically for detecting MMP-2 activity in biological samples, such as culture medium, serum, plasma, synovial fluid, and tissue homogenate, which may contain multiple MMPs. Members of the MMP family have poor substrate sequence specificity, thus using peptide substrate alone to differentiate the activity of a particular MMP from other MMPs is inadequate. A monoclonal anti-human-MMP-2 antibody is therefore employed to pull down MMP-2 from the mixture. MMP-2 activity is then quantified by a 5-FAM/QXL™ 520 fluorescence resonance energy transfer (FRET) peptide (Scheme 1). The long wavelength fluorescence of 5-FAM is less interfered by the autofluorescence of components in biological samples and test compounds. The assay can detect as low as 3 ng/mL active MMP-2 enzyme. The assay has no cross-reactivity with human MMP-1, 3, 7, 8, 9, 10, 12, 13, 15, 16 and has minimal cross-reactivity with MMP-14).

Information d'application

Commentaires:

ELISA + FRET-based Assay Kit

Restrictions: For Research Use only

Stockage

Stock: -80 °C

Stockage commentaire: Store all kit components, except Component B, at -20 °C. Store Component B at -80 °C. For convenience, Components D, G, H can be stored at RT.

Détail du antigène

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Autre désignation: MMP-2

Sujet: Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. MMP-2 (72 kDa gelatinase-A/type IV collagenase) is responsible for degradation of collagen, fibronectin, laminin, and elastin. It has been shown that MMP-2 plays a key role in angiogenesis, tumor cell invasion, and metastasis. MMP-2 is also considered a therapeutic target for cancer.