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FLT3LG Kit ELISA

FLT3LG Reactivité: Humain AA 27-185 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
N° du produit ABIN1889291
  • Antigène Voir toutes FLT3LG Kits ELISA
    FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
    Épitope
    AA 27-185
    Reactivité
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Flt-3ligand
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: T27-P185
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: T27-P185
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    human Flt-3ligand ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Flt-3ligand has been precoated onto 96-well plates. Standards(sf21, T27-P185) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Flt-3ligand is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Flt-3ligand amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human Flt-3ligand standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Flt-3ligand standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 109, Standard deviation: 3.49, CV(%): 3.2
    • Sample 2: n=16, Mean(pg/ml): 256, Standard deviation: 8.45, CV(%): 3.3
    • Sample 3: n=16, Mean(pg/ml): 520, Standard deviation: 22.88, CV(%): 4.4,
    • Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 10.64, CV(%): 9.5
    • Sample 2: n=24, Mean(pg/ml): 245, Standard deviation: 18.38, CV(%): 7.5
    • Sample 3: n=24, Mean(pg/ml): 518, Standard deviation: 41.96, CV(%): 8.1
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes FLT3LG Kits ELISA
    FLT3LG (Fms-Related tyrosine Kinase 3 Ligand (FLT3LG))
    Autre désignation
    FLT3LG (FLT3LG Produits)
    Synonymes
    Flt3lg Kit ELISA, Ly72L Kit ELISA, FL Kit ELISA, FLT3L Kit ELISA, Vegfr3 Kit ELISA, fms related tyrosine kinase 3 ligand Kit ELISA, FMS-like tyrosine kinase 3 ligand Kit ELISA, fms-related tyrosine kinase 4 Kit ELISA, fms-related tyrosine kinase 3 ligand Kit ELISA, FLT3LG Kit ELISA, Flt3l Kit ELISA, Flt4 Kit ELISA, Flt3lg Kit ELISA
    Sujet

    Protein Function: Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins.

    Background: Fms-related tyrosine kinase 3 ligand(FLT3LG), also known as Flt-3ligand or FL, is a protein which in humans is encoded by the FLT3LG gene. It is mapped to 19q13.3. Flt-3ligand is a hematopoietic four helical bundle cytokine. It is structurally homologous to stem cell factor(SCF) and colony stimulating factor 1(CSF-1). In synergy with other growth factors, Flt-3ligand stimulates the proliferation and differentiation of various blood cell progenitors. Besides that, Flt-3ligand can control the development of Dendritic cells(DCs) and is particularly important for plasmacytoid DCs and CD8-positive classical DCs and their CD103-positive tissue counterparts. Flt-3ligand also can enhance the response of stem and primitive progenitor cells to other growth factors to generate all myeloid lineages except erythroid cells.

    Synonyms: Fms-related tyrosine kinase 3 ligand,Flt3 ligand,Flt3L,SL cytokine,FLT3LG,

    Full Gene Name: Fms-related tyrosine kinase 3 ligand

    Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein.
    ID gène
    2323
    UniProt
    P49771
    Pathways
    Signalisation RTK
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