Granulin Kit ELISA

N° du produit ABIN1889363

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris Granulin.

Aperçu rapide pour Granulin Kit ELISA (ABIN1889363)

Antigène: Granulin (GRN)

Épitope: AA 18-589

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit Granulin Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Progranulin

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: T18-L589

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: T18-L589

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Tissue Specificity: Ubiquitous.

Plaque: Pre-coated

Protocole: mouse Progranulin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Progranulin has been precoated onto 96-well plates. Standards(NSO, T18-L589) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Progranulin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Progranulin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Progranulin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse Progranulin standard solution and each sample is measured in duplicate.

Précision du teste: Samnle 1: n=16, Mean(ng/ml): 8.6, Standard deviation: 0.473, CV(%): 5.5 Samnle 2: n=16, Mean(ng/ml): 20, Standard deviation: 1.24, CV(%): 6.2 Samnle 3: n=16, Mean(ng/ml): 31.5, Standard deviation: 1.638, CV(%): 5.2,Samnle 1: n=24, Mean(ng/ml): 9.1, Standard deviation: 0.582, CV(%): 6.4 Samnle 2: n=24, Mean(ng/ml): 25.2, Standard deviation: 1.84, CV(%): 7.3 Samnle 3: n=24, Mean(ng/ml): 36.8, Standard deviation: 2.392, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: Granulin (GRN)

Autre désignation: GRN

Classe de substances: Viral Protein

Sujet:

Protein Function: Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Acts as an autocrine growth factor for PC cells. .

Background: Granulin, also known as Proepithelin(PEPI) is a protein that in humans is encoded by the GRN gene. This gene is mapped to 17q21.31. Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Synonyms: Granulins,PC cell-derived growth factor,PCDGF,Proepithelin,PEPI,Acrogranin,Progranulin,Granulin-1,Granulin-2,Granulin-3,Granulin-4,Granulin-5,Granulin-6,Granulin-7,Grn,

Full Gene Name: Granulins

Cellular Localisation: Secreted.

ID gène: 14824

UniProt: P28798