APCS Kit ELISA

N° du produit ABIN1889377

Kit ELISA Souris APCS, test Colorimetric pour la quantification de Souris APCS.

Aperçu rapide pour APCS Kit ELISA (ABIN1889377)

Antigène: APCS (Amyloid P Component, Serum (APCS))

Épitope: AA 21-224

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Détail du produit APCS Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse SAP/PTX2

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q21-D224

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <50pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q21-D224

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the pentaxin family.

Plaque: Pre-coated

Protocole: mouse SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-D224) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAP amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 1.25 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL mouse SAP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse SAP standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 5.68, Standard deviation: 0.33, CV(%): 5.8
• Sample 2: n=16, Mean(ng/ml): 34, Standard deviation: 1.43, CV(%): 4.2
• Sample 3: n=16, Mean(ng/ml): 61, Standard deviation: 3.05, CV(%): 5,
• Sample 1: n=24, Mean(ng/ml): 5.2, Standard deviation: 0.39, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 39, Standard deviation: 2.11, CV(%): 5.4
• Sample 3: n=24, Mean(ng/ml): 74, Standard deviation: 4.59, CV(%): 6.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: APCS (Amyloid P Component, Serum (APCS))

Autre désignation: APCS

Sujet:

Background: Amyloid P component, serum(SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component(AP), a 25 kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.

Synonyms: Serum amyloid P-component,SAP,Apcs,Ptx2, Sap,

Full Gene Name: Serum amyloid P-component

Cellular Localisation: Secreted.

UniProt: P12246