FCN1 Kit ELISA

N° du produit ABIN1889408

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain FCN1.

Aperçu rapide pour FCN1 Kit ELISA (ABIN1889408)

Antigène: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Épitope: AA 30-326

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Détail du produit FCN1 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Ficolin-1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: A30-A326

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: A30-A326

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the ficolin lectin family.

Tissue Specificity: Peripheral blood leukocytes, monocytes and granulocytes. Also detected in spleen, lung, and thymus, may be due to the presence of tissue macrophages or trapped blood in these tissues. Not detected on lymphocytes. .

Plaque: Pre-coated

Protocole: human Ficolin-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Ficolin-1 has been precoated onto 96-well plates. Standards(NSO, A30-A326) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Ficolin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Ficolin-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 4.83, Standard deviation: 0.188, CV(%): 3.9
• Sample 2: n=16, Mean(ng/ml): 13.2, Standard deviation: 0.581, CV(%): 4.4
• Sample 3: n=16, Mean(ng/ml): 25.1, Standard deviation: 1.431, CV(%): 5.7,
• Sample 1: n=24, Mean(ng/ml): 5.24, Standard deviation: 0.393, CV(%): 7.5
• Sample 2: n=24, Mean(ng/ml): 12.9, Standard deviation: 0.890, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 25.9, Standard deviation: 1.89, CV(%): 7.3

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: FCN1 (Ficolin (Collagen/fibrinogen Domain Containing) 1 (FCN1))

Autre désignation: FCN1

Sujet:

Protein Function: Extracellular lectin functioning as a pattern- recognition receptor in innate immunity. Binds the sugar moieties of pathogen-associated molecular patterns (PAMPs) displayed on microbes and activates the lectin pathway of the complement system. May also activate monocytes through a G protein-coupled receptor, FFAR2, inducing the secretion of interleukin-8/IL-8 (PubMed:21037097). Binds preferentially to 9-O-acetylated 2-6- linked sialic acid derivatives and to various glycans containing sialic acid engaged in a 2-3 linkage. .

Background: Ficolin-1, and also commonly termed M-ficolin is a protein that in humans is encoded by the FCN1 gene. It is mapped to 9q34.3. The ficolin family of proteins are characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. Ficolins have a crucial role in defense against pneumococcal infection through the lectin complement pathway. Ficolin 1 is predominantly expressed in the peripheral blood leukocytes, and has been postulated to function as a plasma protein with elastin-binding activity.

Synonyms: Ficolin-1,Collagen/fibrinogen domain-containing protein 1,Ficolin-A,Ficolin-alpha,M-ficolin,FCN1,FCNM,

Full Gene Name: Ficolin-1

Cellular Localisation: Secreted. Cell membrane, Peripheral membrane protein, Extracellular side. Found on the monocyte and granulocyte surface.

ID gène: 2219

UniProt: O00602

Pathways: Système du Complément