DLL1 Kit ELISA

N° du produit ABIN1889418

Ce kit ELISA Humain DLL1 est un kit ELISA Colorimetric conçu pour quantifier Humain DLL1.

Aperçu rapide pour DLL1 Kit ELISA (ABIN1889418)

Antigène: DLL1 (delta-Like 1 (DLL1))

Épitope: AA 22-540

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78-5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Urine, Milk

Détail du produit DLL1 Kit ELISA

Seuil minimal de détection: 78 pg/mL

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DLL1

Marque: PicoKine™

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: S22-G540

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: S22-G540

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 DSL domain.

Tissue Specificity: Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.

Plaque: Pre-coated

Protocole: human DLL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DLL1 has been precoated onto 96-well plates. Standards(NSO, S22-G540) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DLL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DLL1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human DLL1 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), urine or human milk to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each human DLL1 standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 713, Standard deviation: 24.9, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 1579, Standard deviation: 64.74, CV(%): 4.1
• Sample 3: n=16, Mean(pg/ml): 3618, Standard deviation: 137.5, CV(%): 3.8,
• Sample 1: n=24, Mean(pg/ml): 674, Standard deviation: 49.8, CV(%): 7.4
• Sample 2: n=24, Mean(pg/ml): 1486, Standard deviation: 101, CV(%): 6.8
• Sample 3: n=24, Mean(pg/ml): 3542, Standard deviation: 269.2, CV(%): 7.6

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: DLL1 (delta-Like 1 (DLL1))

Autre désignation: DLL1

Sujet:

Protein Function: Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. .

Background: DLL1(DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Synonyms: Delta-like protein 1,Drosophila Delta homolog 1,Delta1,H-Delta-1,DLL1,UNQ146/PRO172,

Full Gene Name: Delta-like protein 1

Cellular Localisation: Membrane, Single-pass type I membrane protein.

ID gène: 28514

UniProt: O00548

Pathways: Signalisation Notch, Stem Cell Maintenance