Chitinase 3-Like 1/YKL-40 Kit ELISA
Aperçu rapide pour Chitinase 3-Like 1/YKL-40 Kit ELISA (ABIN2042406)
Antigène
Reactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 62.5 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-Chitinase 3-like 1/YKL-40, Mouse
Lyophilized recombinant Chitinase 3-like 1/YKL-40, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Mouse Chitinase 3-like 1 /YKL-40 in cell culture supernates, serum and plasma (heparin, EDTA).Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40kD in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- Chitinase 3-Like 1/YKL-40
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ID gène
- 12654
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UniProt
- Q61362
Antigène
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