GZMB Kit ELISA
Aperçu rapide pour GZMB Kit ELISA (ABIN2042488)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 15.6 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-Granzyme B, Human
Lyophilized recombinant Granzyme B, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Human Granzyme B in cell culture supernates, serum and plasma (heparin, EDTA).Granzyme B is a serine protease that in humans is encoded by the GZMB gene. Granzyme B is expressed by cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. CTL and NK cells share the remarkable ability to recognize specific infected target cells. They are thought to protect their host by inducing apoptosis of cells that bear on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein encoded by this gene is crucial for the rapid induction of target cell apoptosis by CTL in cell-mediated immune response.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- GZMB (Granzyme B (GZMB))
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Autre désignation
- Granzyme B
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ID gène
- 3002
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UniProt
- P10144
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Pathways
- Apoptose, Caspase Cascade in Apoptosis
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