HSP27 Kit ELISA

N° du produit ABIN2042492

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain HSP27.

Aperçu rapide pour HSP27 Kit ELISA (ABIN2042492)

Antigène: HSP27 (Heat Shock Protein 27 (HSP27))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Application: ELISA

Détail du produit HSP27 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients: 96-well plate pre-coated with anti-HSP27, Human
Lyophilized recombinant HSP27, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Human HSP27 in cell lysates.Hsp27 is a chaperone of the sHsp (small heat shock protein) group among ubiquitin, -crystallin, Hsp20 and others. The common functions of sHsps are chaperone activity, thermotolerance, inhibition of apoptosis, regulation of cell development, and cell differentiation. They also take part in signal transduction. The HSP27 gene has 3 exons. The mouse Hsp25 gene was mapped to chromosome 5 in a region homologous to 7q in the human. They also mapped the mouse Hsp105 gene to chromosome 5 but suggested that the human homolog is probably on 13q, not chromosome 7. The standard used in this kit is recombinant human HSP27, consisting of 205 amino acids with the molecular mass of 22.7kD.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HSP27 (Heat Shock Protein 27 (HSP27))

ID gène: 3315

UniProt: P04792

Pathways: VEGF Signaling