IL-4 Kit ELISA

N° du produit ABIN2042554

Kit ELISA IL-4 Rat, Colorimetric test pour la quantification de Rat IL-4.

Aperçu rapide pour IL-4 Kit ELISA (ABIN2042554)

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Détail du produit IL-4 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients: 96-well plate pre-coated with anti-IL-4, Rat
Lyophilized recombinant IL-4, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Rat IL-4 in cell culture supernates and serum.Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.1 The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis.1 The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5.2 Rat IL-4 is a 13.5kD glycoprotein, consisting of 147 amino acids in precursor form and 123 amino acids in mature form.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL-4 (IL4) (Interleukin 4 (IL4))

Autre désignation: IL-4

ID gène: 287287

UniProt: P20096

Pathways: Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation