M-CSF/CSF1 Kit ELISA
Aperçu rapide pour M-CSF/CSF1 Kit ELISA (ABIN2042584)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 15.6 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 1 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-M-CSF, Mouse
Lyophilized recombinant M-CSF, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Mouse M-CSF in cell culture supernates, tissue homogenates, serum and plasma (EDTA).M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))
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Autre désignation
- M-CSF
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ID gène
- 12977
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UniProt
- P07141
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Pathways
- Signalisation RTK
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