CCL20 Kit ELISA

N° du produit ABIN2042598

Ce kit ELISA Humain CCL20 est un kit ELISA Colorimetric conçu pour quantifier Humain CCL20.

Aperçu rapide pour CCL20 Kit ELISA (ABIN2042598)

Antigène: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Détail du produit CCL20 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients: 96-well plate pre-coated with anti-MIP-3 alpha/CCL20, Human
Lyophilized recombinant MIP-3 alpha/CCL20, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Human MIP-3 alpha in cell culture supernates, serum and plasma (heparin, EDTA).Macrophage Inflammatory Protein 3 (MIP3 ), also called Chemokine, cc motif, ligand 20 (CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3 is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3 /CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3 and Serine MIP-3 , the two forms of MIP3 , that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3 .

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Autre désignation: MIP-3 alpha/ccl20

ID gène: 6364

UniProt: P78556

Pathways: Phosphorylation & l'infection par le SRAS-CoV-2