MMP2 Kit ELISA

N° du produit ABIN2042604

Ce kit ELISA Rat MMP2 est un kit ELISA Colorimetric conçu pour quantifier Rat MMP2.

Aperçu rapide pour MMP2 Kit ELISA (ABIN2042604)

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 6-10000 pg/mL

Application: ELISA

Détail du produit MMP2 Kit ELISA

Seuil minimal de détection: 6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate pre-coated with anti-MMP-2, Rat
Lyophilized recombinant MMP-2, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Rat MMP-2 in cell culture supernates, serum, plasma (heparin).Type IV collagenase, 72kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72kD. The detected MMP-2 includes zymogen and active enzyme.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Autre désignation: MMP-2

ID gène: 81686

UniProt: P33436

Pathways: Activation of Innate immune Response