CCL5 Kit ELISA

N° du produit ABIN2042650

Kit ELISA Souris CCL5, test Colorimetric pour la quantification de Souris CCL5.

Aperçu rapide pour CCL5 Kit ELISA (ABIN2042650)

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Détail du produit CCL5 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients: 96-well plate pre-coated with anti-Rantes, Mouse
Lyophilized recombinant Rantes, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Information d'application

Plaque: Pre-coated

Protocole: Sandwich ELISA quantitative detection kit for Mouse Rantes in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10kD and, after cleavage of the signal peptide, approximately 8kD. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CCL5 (Chemokine (C-C Motif) Ligand 5 (CCL5))

Autre désignation: Rantes

ID gène: 20304

UniProt: P30882

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration