LTA Kit ELISA
Aperçu rapide pour LTA Kit ELISA (ABIN2042691)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 62.5 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 5 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-TNF beta, Human
Lyophilized recombinant TNF beta, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Human TNF beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35% identity and 50% homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8kD.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- LTA (Lymphotoxin-alpha (LTA))
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Autre désignation
- TNF beta
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Classe de substances
- Chemical
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ID gène
- 4049
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UniProt
- P01374
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Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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