LTA Kit ELISA

N° du produit ABIN411381

Le kit ELISA LTA (ABIN411381) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain LTA. Ce kit ELISA a été cité dans 2 publications.

Aperçu rapide pour LTA Kit ELISA (ABIN411381)

Antigène: LTA (Lymphotoxin-alpha (LTA))

Épitope: AA 35-205

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Détail du produit LTA Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

Fonction: Sandwich Human TNF Beta ELISA Kit to quantitate Human LTA in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: L35-L205

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7.1%, 6.6%, 7.1%
Inter-Assay Precision (CV%): 7.3%, 7.4%, 8.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour LTA Kit ELISA (ABIN411381)

Wang, Zheng, Meng, Wang, He, Zhang, Liu, Hu, He, Hu, Wang: "In vivo study of immunogenicity and kinetic characteristics of a quantum dot-labelled baculovirus." dans: Biomaterials, Vol. 64, pp. 78-87, (2015) (PubMed).

Zeng, Huang, Zheng, Guo, Pan: "Effects of reactive nitrogen scavengers on NK-cell-mediated killing of K562 cells." dans: Journal of biomedicine & biotechnology, Vol. 2012, pp. 101737, (2012) (PubMed).

Détail du antigène

Antigène: LTA (Lymphotoxin-alpha (LTA))

Autre désignation: LTA

Classe de substances: Chemical

Sujet:

Background: Tumor necrosis factor-beta (TNF-beta), previously called lymphotoxin-alpha (LTA), is a cytokine produced by lymphocytes. TNF-alpha and TNF-beta share 35 % identity and 50 % homology in the amino acid sequence. The substance LTA mediates a wide variety of inflammatory, immunostimulatory, and antiviral responses. LTA is associated with susceptibility to myocardial infarction, asthma and other diseases. The LTA gene is located on human chromosome 6. The standard product used in this kit is recombinant protein, consisting of 172 amino acids with the molecular mass of 18.8KDa.

Gene Full Name: lymphotoxin alpha

ID gène: 4049

UniProt: P01374

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process