VEGF Kit ELISA
Aperçu rapide pour VEGF Kit ELISA (ABIN2042720)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 15.6 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 2 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-VEGF, Mouse
Lyophilized recombinant VEGF, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Mouse VEGF in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias.VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy.VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42kD dimer.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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Autre désignation
- VEGF
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ID gène
- 22339
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UniProt
- Q00731
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