VEGFC Kit ELISA
Aperçu rapide pour VEGFC Kit ELISA (ABIN2042722)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 62.5 pg/mL
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Réactivité croisée (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 3 pg/mL
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Ingrédients
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96-well plate pre-coated with anti-VEGF-C, Human
Lyophilized recombinant VEGF-C, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plaque
- Pre-coated
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Protocole
- Sandwich ELISA quantitative detection kit for Human VEGF-C in cell culture supernates, serum and plasma (heparin, EDTA).Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23kD after glycosylation.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- VEGFC (Vascular Endothelial Growth Factor C (VEGFC))
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Autre désignation
- VEGF-C
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ID gène
- 7424
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UniProt
- P49767
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Pathways
- Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2
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