ACAT1 Kit ELISA

N° du produit ABIN2042749

Détail du produit ACAT1 Kit ELISA

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Marque: BioAssay™

Sensibilité: < 0.052 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 1 (ACAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Autre désignation: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) (ACAT1 Produits)

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