Acetyl-CoA Carboxylase alpha Kit ELISA

N° du produit ABIN2042755

Kit ELISA Rat Acetyl-CoA Carboxylase alpha, test Colorimetric pour la quantification de Rat Acetyl-CoA Carboxylase alpha.

Aperçu rapide pour Acetyl-CoA Carboxylase alpha Kit ELISA (ABIN2042755)

Antigène: Acetyl-CoA Carboxylase alpha (ACACA)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12-200 ng/mL

Application: ELISA

Détail du produit Acetyl-CoA Carboxylase alpha Kit ELISA

Seuil minimal de détection: 3.12 ng/mL

Sensibilité: < 1.34 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Acetyl Coenzyme A Carboxylase Alpha (ACACa) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Carboxylase Alpha (ACACa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Carboxylase Alpha (ACACa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Carboxylase Alpha (ACACa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: Acetyl-CoA Carboxylase alpha (ACACA)

Autre désignation: Acetyl Coenzyme A Carboxylase alpha (ACACa)

Pathways: AMPK Signaling, Ribonucleoside Biosynthetic Process, L'effet Warburg