APOA1 Kit ELISA

N° du produit ABIN2042917

Kit ELISA APOA1 Chien, Colorimetric test pour la quantification de Chien APOA1.

Aperçu rapide pour APOA1 Kit ELISA (ABIN2042917)

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 ng/mL

Application: ELISA

Détail du produit APOA1 Kit ELISA

Seuil minimal de détection: 31.25 ng/mL

Sensibilité: < 14.8 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Apolipoprotein A1 (APOA1) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Autre désignation: Apolipoprotein A1 (APOA1)

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism