APOA5 Kit ELISA

N° du produit ABIN2042925

Ce kit ELISA Rat APOA5 est un kit ELISA Colorimetric conçu pour quantifier Rat APOA5.

Aperçu rapide pour APOA5 Kit ELISA (ABIN2042925)

Antigène: APOA5 (Apolipoprotein A-V (APOA5))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit APOA5 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.059 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Apolipoprotein A5 (APOA5) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A5 (APOA5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A5 (APOA5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A5 (APOA5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A5 (APOA5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: APOA5 (Apolipoprotein A-V (APOA5))

Autre désignation: Apolipoprotein A5 (APOA5)

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism