APOE Kit ELISA

N° du produit ABIN2042927

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Chien APOE.

Aperçu rapide pour APOE Kit ELISA (ABIN2042927)

Antigène: APOE (Apolipoprotein E (APOE))

Reactivité: Chien

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.39-25 ng/mL

Application: ELISA

Détail du produit APOE Kit ELISA

Seuil minimal de détection: 0.39 ng/mL

Sensibilité: < 0.17 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Apolipoprotein E (APOE) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein E (APOE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein E (APOE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein E (APOE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein E (APOE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: APOE (Apolipoprotein E (APOE))

Pathways: Regulation of Cell Size, Lipid Metabolism