BCL2L1 Kit ELISA
Aperçu rapide pour BCL2L1 Kit ELISA (ABIN2042991)
Antigène
Voir toutes BCL2L1 Kits ELISAReactivité
Méthode de détection
Type de méthode
Gamme de detection
Application
-
-
Seuil minimal de détection
- 7.81 pg/mL
-
Sensibilité
- < 3.3 pg/mL
-
-
-
-
Plaque
- Pre-coated
-
Protocole
- B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-
Restrictions
- For Research Use only
-
-
-
Stock
- 4 °C/-20 °C
-
-
- BCL2L1 (BCL2-Like 1 (BCL2L1))
-
Autre désignation
- B-Cell CLL/Lymphoma 2 Like Protein (Bcl2L)
-
Pathways
- Apoptose, Negative Regulation of intrinsic apoptotic Signaling
Antigène Voir toutes BCL2L1 Kits ELISA
-