CrkL Kit ELISA

N° du produit ABIN2043245

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris CrkL.

Aperçu rapide pour CrkL Kit ELISA (ABIN2043245)

Antigène: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.12-5000 pg/mL

Application: ELISA

Détail du produit CrkL Kit ELISA

Seuil minimal de détection: 78.12 pg/mL

Sensibilité: < 33 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Crk Like Protein (CRKL) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Crk Like Protein (CRKL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Crk Like Protein (CRKL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Crk Like Protein (CRKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Crk Like Protein (CRKL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Autre désignation: Crk Like Protein (CRKL)

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor