DKK4 Kit ELISA

N° du produit ABIN2043341

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris DKK4.

Aperçu rapide pour DKK4 Kit ELISA (ABIN2043341)

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit DKK4 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.051 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Dickkopf Related Protein 4 (DKK4) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dickkopf Related Protein 4 (DKK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dickkopf Related Protein 4 (DKK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dickkopf Related Protein 4 (DKK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dickkopf Related Protein 4 (DKK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Autre désignation: Dickkopf Related Protein 4 (DKK4)

Pathways: Signalisation WNT