RAD50 Kit ELISA

N° du produit ABIN2043361

Kit ELISA RAD50 Humain, Colorimetric test pour la quantification de Humain RAD50.

Aperçu rapide pour RAD50 Kit ELISA (ABIN2043361)

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit RAD50 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.057 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: DNA Repair Protein RAD50 (RAD50) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNA Repair Protein RAD50 (RAD50). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DNA Repair Protein RAD50 (RAD50). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNA Repair Protein RAD50 (RAD50), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of DNA Repair Protein RAD50 (RAD50) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Autre désignation: DNA Repair Protein RAD50 (RAD50)

Pathways: Réparation de l'ADN, Protein targeting to Nucleus