FGF21 Kit ELISA

N° du produit ABIN2043473

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris FGF21.

Aperçu rapide pour FGF21 Kit ELISA (ABIN2043473)

Antigène: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.81-500 pg/mL

Application: ELISA

Détail du produit FGF21 Kit ELISA

Seuil minimal de détection: 7.81 pg/mL

Sensibilité: < 3.2 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Fibroblast Growth Factor 21 (FGF21) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Pathways: Signalisation RTK