GLB1 Kit ELISA

N° du produit ABIN2043529

Kit ELISA Humain GLB1, test Colorimetric pour la quantification de Humain GLB1.

Aperçu rapide pour GLB1 Kit ELISA (ABIN2043529)

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit GLB1 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.116 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Galactosidase Beta (GLb) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galactosidase Beta (GLb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galactosidase Beta (GLb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galactosidase Beta (GLb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Galactosidase Beta (GLb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: GLB1 (Galactosidase, beta 1 (GLB1))

Autre désignation: Galactosidase beta (GLb)

Pathways: Glycosaminoglycan Metabolic Process