GAD Kit ELISA

N° du produit ABIN2043559

Kit ELISA Souris GAD, test Colorimetric pour la quantification de Souris GAD.

Aperçu rapide pour GAD Kit ELISA (ABIN2043559)

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 ng/mL

Application: ELISA

Détail du produit GAD Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Sensibilité: < 0.31 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Glutamate Decarboxylase 1, Brain (GAD1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Decarboxylase 1, Brain (GAD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Decarboxylase 1, Brain (GAD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Decarboxylase 1, Brain (GAD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutamate Decarboxylase 1, Brain (GAD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Autre désignation: Glutamate Decarboxylase 1, Brain (GAD1)