HDGF Kit ELISA

N° du produit ABIN2043637

Kit ELISA Souris HDGF, test Colorimetric pour la quantification de Souris HDGF.

Aperçu rapide pour HDGF Kit ELISA (ABIN2043637)

Antigène: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 pg/mL

Application: ELISA

Détail du produit HDGF Kit ELISA

Seuil minimal de détection: 31.25 pg/mL

Marque: BioAssay™

Sensibilité: < 13.8 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Hepatoma Derived Growth Factor (HDGF) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hepatoma Derived Growth Factor (HDGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hepatoma Derived Growth Factor (HDGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hepatoma Derived Growth Factor (HDGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Hepatoma Derived Growth Factor (HDGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HDGF (Hepatoma-Derived Growth Factor (HDGF))

Autre désignation: Hepatoma Derived Growth Factor (HDGF)

Pathways: ER-Nucleus Signaling