IL1A Kit ELISA

N° du produit ABIN2043671

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat IL1A.

Aperçu rapide pour IL1A Kit ELISA (ABIN2043671)

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78-50 pg/mL

Application: ELISA

Détail du produit IL1A Kit ELISA

Seuil minimal de détection: 0.78 pg/mL

Sensibilité: < 0.21 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: High Sensitivity Interleukin 1 Alpha (IL1a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Alpha (IL1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 1 Alpha (IL1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Alpha (IL1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 1 Alpha (IL1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: IL1A (Interleukin 1 alpha (IL1A))

Pathways: Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints